This is a 31.5 mile route beginning in Fort White, FL. It travels down the grade-separated asphalt Ichetucknee - O'Leno State Trail, connecting Ichetucknee Springs State Park to O'leno State Park, and is one of the few bicycle trails which connects two state parks.
This route starts just north of Fort White, at the library. In Fort White, there are several local restaurants that riders can enjoy. The trail then travels east across U.S. 441 to O'leno State Park & River Rise Preserve State Park. The trail completes a 3.5 mile loop in the scenic state park, then continues back towards Fort White. Riders then travel all the way to beginning of the Ichetucknee River Greenway, traveling on it for a short time along Ichetucknee Springs State Park to the river. Riders then return along the same trail to Fort White.